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| Cell line: | AP-1060 |
| DSMZ no.: | ACC 593 |
| Species: | human (Homo sapiens) |
| Cell type: | acute myeloid leukemia |
| Origin: | established from the bone marrow of a 45-year-old man with acute promyelocytic leukemia (APL = AML FAB M3) in fourth relapse in 1998; cells were immortalized using ethyl-nitrosourea; cells are described in the literature to be constitutively dependent on GM-CSF and IL-3 or G-CSF; cells carry and express the t(15;17)(q22;q11.2) PML-RARA fusion gene and the t(12;15)(p13;q25) ETV6-NTRK3 fusion gene |
| Reference(s): | 14994, 18148 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| Depositor-specific restrictions: | Transfer to third parties needs to be negotiated with the depositor. |
| | DSMZ Cell Culture Data: |
| Morphology: | single round cells growing in suspension; image; image; image |
| Medium: | 70% Iscove's MDM + 20% h.i. FBS + 10% vol conditioned medium of cell line 5637 (DSMZ ACC 35) |
| Subculture: | cells are difficult to culture! Seed out at ca. 1 x 106 cells/ml in a 24-well-plate; split saturated culture 1:2 or less once or twice a week; maintain at ca. 1.0-1.5 x 106 cells/ml; use always 24- or 12-well-plates |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 80 hours |
| Harvest: | maximum density at about 2.5 x 106 cells/ml |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in microbiological culture, PCR assays |
| Immunology: | CD3-, CD4+, CD13(+), CD14-, CD15(+), CD19-, CD33+, CD34(+), HLA-DR-, CD68+, cCD3-; image |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human near-diploid karyotype with 3% polyploidy - 46(42-47)<2n>XY, t(3;14)(p21;q11.2), add(5)(q33), del(6)(q25), add(12)(p13), t(15;17)(q22;q21); carries t(15;17) with rearrangement of PML and RARA; resembles published karyotype |
| Molec. Genetics: | expression of fusion gene PML-RARA (L-form) confirmed by RT-PCR |
| Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
