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| Cell line: | T2 |
| DSMZ no.: | ACC 598 |
| Species: | human-human (Homo sapiens) |
| Cell type: | somatic cell hybrid |
| Origin: | established by PEG-mediated fusion of the B-lymphoblastoid cell line (B-LCL) LCL 721.174 with an 8-azaguanine and ouabain-resistant variant of the T-LCL CEM (CEMR.3); cells were described in the literature to synthesize HLA-A2 and -B5 RNA, but express only small amounts of A2 and no B5 antigen on the cell surface; subclone of the T1 cell line which has lost both CEMR-derived copies of chromosome 6; cell line also known as 174xCEM.T2 |
| Reference(s): | 14914, 14915 |
| Biosafety level: | 1 |
| Risk assessment: | The cell line is positive for EBV (HHV-4) by PCR analysis. However, expression of immediate-early protein BZLF-1 and lately expressed capsid protein were negative by western blot and immunostaining, respectively, in untreated and phorbol ester / sodium butyrate stimulated cells. The infection was classified as latent infection without production of active viruses. A transmission of EBV during handling of the cells is unlikely and thus the cell line is categorized biosafety level 1. According to a recommendation of the German Central Committee for Biological Safety (ZKBS) the cells have to be handled under biosafety level 2 when the cells are used in genetic engineering projects. |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | round to polymorphic cells growing in suspension singly and in clumps; image; image |
| Medium: | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture: | seed out at ca. 0.2-1 x 106 cells/ml; split culture 1:2 to 1:4 every 2-3 days; maximal density at about 1.5 x 106 cells/ml |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 40-60 hours |
| Harvest: | cell harvest of ca. 1 x 106 cells/ml |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in microbiological culture, PCR assays |
| Immunology: | CD2 -, CD3 -, CD4 +, CD5 -, CD6 -, CD7 +, CD8 -, CD10 -, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 -, CD138 -, TCRalpha/beta -, TCRgamma/delta -, HLA-DR -; image |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human flat-moded hypertriploid karyotype with 5% polyploidy; 69-78<3n>XX, +2, +11, +13, +14, +16, +20, +20, +21, +mar, add(X)(q11), del(7)(q31.3), add(8)(p11), t(8;9)(p11;p24), del(9)(q21)x1-2, add(14)(q32)x1-2, add(16)(q11), add(18)(q23); derived from CCRF-CEM |
| Viruses: | PCR: EBV +, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
