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| Cell line: | BEN-MEN-1 |
| DSMZ no.: | ACC 599 |
| Species: | human (Homo sapiens) |
| Cell type: | benign meningioma |
| Origin: | established in 2003 from the meningothelial meningioma (grade I, WHO, #1238-03) attached to the parietal falx of a 68-year-old woman after surgical tumor resection; cells were immortalized by retroviral transduction with human telomerase reverse transcriptase (hTERT) |
| Reference(s): | 14864 |
| Biosafety level: | 1, GMO-S1 |
| Permissions and restrictions: | A |
| Depositor-specific restrictions: | Transfer to third parties needs to be negotiated with the depositor. |
| | DSMZ Cell Culture Data: |
| Morphology: | adherent, epithelioid cells growing as monolayers; image; image; image; image |
| Medium: | 80% DMEM + 20% h.i. FBS |
| Subculture: | seed out at ca. 0.7-2.0 x 106 cells/175 cm2; optimal split ratio 1:2 to 1:5 every 2-6 days using trypsin/EDTA |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 40-50 hours |
| Harvest: | cell harvest of ca. 10 x 106 cells/175 cm2 |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in microbiological culture, PCR assays |
| Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human hypodiploid karyotype with 12% polyploidy; 44(43-46)<2n>XX, -22; frequent random chromosome loss; monosomy 22 is recurrent in grade I benign meningiomas; matches published karyotype |
| Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
