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Cell line: | SUP-T11 |
DSMZ no.: | ACC 605 |
Species: | human (Homo sapiens) |
Cell type: | T cell leukemia |
Origin: | derived from the bone marrow of a 74-year-old man with T-ALL at diagnosis; cell line was established in the presence of IGF-I under hypoxic conditions; cells were described in the literature to express high levels of TCL1A and TCL1B genes due to their juxtaposition from chromosome 14q32.1 to the regulatory element of the T-cell receptor C alpha/delta locus at chromosome 14q11 |
Reference(s): | 14971, 14993, 15011, 15045 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | small, polymorph cells growing singly in suspension; image; image; image |
Medium: | 90% RPMI 1640 + 10% h.i. FBS |
Subculture: | seed out at ca. 0.5 x 106 cells/ml; optimal split ratio of 1:2 to 1:4 every 2-3 days; maintain at ca. 0.2-1.5 x 106 cells/ml, at first we recommend to use a 24-well plate |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | ca. 35-45 hours |
Harvest: | maximum density at ca. 1.5 x 106 cells/ml |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in microbiological culture, PCR assays |
Immunology: | CD2 +, CD3 +, CD4 -, CD5 +, CD6 +, CD7 +, CD8 -, CD13 -, CD19 -, CD20 -, CD34 -, HLA-DR -; image |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Cytogenetics: | human highly rearranged hypodiploid karyotype with 5% polyploidy; 38(36-39)<2n>XX, -Y, -10, -13, -15, -16, -17, -21, -22, del(X)(q11?q23), add(X)(q23), der(1)del(1)(p32)t(1;4)(q32;q32.2), der(1)t(1;7)(q32;q32), der(2)t(2;4)(p23;p15 or q35), der(3)t(1;3)(p33;q28), der(4)t(1;4)(p32;q32.2), dup(4)(p11p14), der(5)t(5;9)(p15;q11.2), der(6)t(1;6)(p3?2;p2?4), add(6)(q27), der(7)t(7;15)(q31.2;q21.2), add(8)(q24.33), der(8)t(8;16)(p12;p13), del(9)(q21q23), der(12)t(8;12)(q24;p13)t(12;16)(q24;23), t(14;14)(q11.2;q32.1), der(15)t(1;15)(q31;q26), der(19)t(19;21)(p13;q21), der(20)t(17;20)(p11;q13), der(21)t(12;21)(?q24;q12), add(22)(q13); resembles published karyotype; t(14;14) effects activation of TCL1 by juxtaposition with T-cell receptor A/D locus |
Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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