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Cell line: | HAL-01 |
DSMZ no.: | ACC 610 |
Species: | human (Homo sapiens) |
Cell type: | B cell precursor leukemia |
Origin: | established from the peripheral blood of a 17-year-old woman with acute lymphoblastic leukemia (ALL-L2) in 1990; contains the t(17;19)(q22;p13) TCF3-HLF (E2A-HLF) fusion gene |
Reference(s): | 14744, 14820, 18196 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | single, round cells in suspension, some cells are slightly adherent; image; image; image; image |
Medium: | 90% RPMI 1640 + 10% h.i. FBS |
Subculture: | seed out at ca. 1 x 106 cells/ml; maintain at 1-2 x 106 cells/ml; optimal split ratio of 1:2 to 1:3 twice a week; adherent cells can be detached by tapping the flask |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | ca. 48 hours |
Harvest: | maximum density at ca. 3 x 106 cells/ml |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in microbiological culture, PCR assays |
Immunology: | CD3-, CD10+, CD13-, CD19+, CD20(+), CD34-, CD37+, CD38+, CD80-, CD138+, HLA-DR+, sIgkappa-, cIgkappa-, sIglambda-, cIgkappa-, sIgG-, cIgG-, sIgM-, cIgM-; image |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Cytogenetics: | human near-diploid karyotype with 4% polyploidy;
46(43-48)<2n>XX, t(1;7)(p34;q21), t(17;19)(q22;p13); t(17;19) effects rearrangement of TCF3 (E2A) and HLF; matches published karyotype |
Molec. Genetics: | expression of fusion gene TCF3-HLF (E2A-HLF) confirmed by RT-PCR |
Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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