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| Cell line: | RL |
| DSMZ no.: | ACC 613 |
| Species: | human (Homo sapiens) |
| Cell type: | B cell lymphoma |
| Origin: | established in 1983 from the ascites of a 52-year-old man with B-NHL (diffuse, undifferentiated, small non-cleaved large cell) |
| Reference(s): | 14375 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | lymphoid cells growing singly or in clumps in suspension; image; image; image |
| Medium: | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture: | seed out at ca. 1 x 106 cells/ml; maintain at 0.7-1.5 x 106 cells/ml; optimal split ratio of 1:2 to 1:3 every 2-5 days |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 5-8 days |
| Harvest: | maximum density at ca. 1.8 x 106 cells/ml |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in microbiological culture, PCR assays |
| Immunology: | CD3 -, CD10 +, CD19 +, CD20 +, CD37 +, CD38 +, cyCD79a +, HLA-DR +, sm/cyIgG -, sm/cyIgM +, sm/cykappa -, sm/cylambda +; image |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human hyperdiploid karyotype with 16% polyploidy; 49(45-51)<2n>XY, +7, +17, +21, der(3)inv(3)(p24q24-q25)t(3;13)(q24-q25;q32), add(5)(p11), der(13)t(3;13)(q24-q25;q32), t(14;18)(q32;q21), der(17)dup(17)(p12p13)t(17;20)(q11.2;q11.2), r(17), add(19)(q12), der(19)t(19;20)(p13;p11), der(20)t(17;20)(q11.2;q11.2)x1-2; carries t(14;18) effecting rearrangement of BCL2 with IGH (IGH); resembles published karyotype |
| Molec. Genetics: | expression of fusion gene IGH-BCL2 confirmed by RT-PCR |
| Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
