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| Cell line: | MEL-HO |
| DSMZ no.: | ACC 62 |
| Species: | human (Homo sapiens) |
| Cell type: | melanoma |
| Origin: | established from the primary tumor of a woman with melanoma in 1976 |
| Reference(s): | 14615, 15210 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | epithelial-like adherent cells with long, narrow, often branching cell bodies; image; image; image; image; image; image; image |
| Medium: | 95% RPMI 1640 + 5% h.i. FBS |
| Subculture: | seed out at ca. 1 x 106 cells/80 cm2 or 175 cm2; split confluent culture 1:5 to 1:10 using trypsin/EDTA |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 24 hours |
| Harvest: | about 10-12 x 106 cells/80 cm2 |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human hypertriploid karyotype with 1.5% polyploidy - 74(72-76)<3n>XX, -X, +3, +7, +7, -8, -10, -11, +15, -16, +17, +18, +20, +21, +mar - del(1)(q42), del(3)(p21), i(7q)x2, del(9)(p13), der(15)t(15;?)(q21;?), i(17q), del(X)(q11) |
| Molec. Genetics: | Genomic sequencing and/or ARMS assay show that the cell line carries the BRAF V600E mutation (mu/wt), but no NRAS Q61 mutation |
| Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
