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Cell line: | U-HO1 |
DSMZ no.: | ACC 626 |
Species: | human (Homo sapiens) |
Cell type: | Hodgkin lymphoma |
Origin: | established in 2005 from the pleural effusion of a 23-year-old man with terminal refractory classical Hodgkin lymphoma (nodular sclerosing type) after radiotherapy, salvage chemotherapy and autologous stem cell transplantation; described in the literature to carry an amplification of REL-BCL11A region with overexpression of miR-216/217 |
Reference(s): | 14728, 40211 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
Depositor-specific restrictions: | Transfer to third parties needs to be negotiated with the depositor. |
| DSMZ Cell Culture Data: |
Morphology: | polymorphic cells growing singly and in clumps in suspension; occasionally large cells present; image |
Medium: | 80% Iscove's MDM + RPMI 1640 (at 4:1) + 20% h.i. FBS, 2 mM L-glutamine |
Subculture: | seed out at ca. 1-1.5 x 106 cells/well in a 24-well-plate; optimal split ratio 1:2 every 3-5 days |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | ca. 100 hours |
Harvest: | maximum density at ca. 2 x 106 cells/ml |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in microbiological culture, PCR assays |
Immunology: | CD2 -, CD3 -, CD13 -, CD14 -, CD15 +, CD19 -, CD21 -, CD25 -, CD30 +, CD34 -, HLA-DR +; image |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Cytogenetics: | human hyperdiploid karyotype with 9% polyploidy; 51(46-52)<2n>XY, +5, +6, +8, +12, +17, +20, -22, +mar, del(1)(p13p31), der(2)qdp(2)(p1?5p1?6)t(2;10)(p24;q25.2), add(4)(q35), t(4;6)(p15.2;p21), del(5)(q11q34), der(5)t(5;15)(p11;q13), t(5;22)(q35;q13), del(6)(q13), der(10)t(2;10)(p24;q25.2)x1-2, t(12;18)(p10;q10), del(15)(q11q14), add(15)(q14), del(20)(q13)x1-2, dup(21)(q22.1q22.2), der(21)t(15;21)(q15;p11); genomic amplification of BCL11A-REL region on der(2); resembles published karyotype. |
Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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