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Cell line: | SK-N-BE(2) |
DSMZ no.: | ACC 632 |
Species: | human (Homo sapiens) |
Cell type: | neuroblastoma |
Origin: | established in 1972 from the bone marrow biopsy of a 22-month-old boy with disseminated neuroblastoma after repeated courses of chemotherapy and radiotherapy; described to carry mutations in the TP53 gene |
Reference(s): | 14389, 14390, 14391 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
Depositor-specific restrictions: | Transfer to third parties needs to be negotiated with the depositor. |
| DSMZ Cell Culture Data: |
Morphology: | epitheloid cells growing adherently in monolayers; image; image |
Medium: | 90% mixture of Ham's F12 + MEM (with Earle's salts)(at 1:1) + 10% h.i. FBS |
Subculture: | seed out at ca. 1-2 x 106 cells/80 cm2; split confluent culture 1:6 - 1:10 every week using trypsin/EDTA |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | ca. 36 hours |
Harvest: | maximum density at ca. 10 x 106 cells/80 cm2 |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in microbiological culture, PCR assays |
Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Cytogenetics: | human hypodiploid karyotype with 12% polyploidy
43<2n>(42-49)X, -Y, +6, -9, -17, -18, der(1)t(1;2)(p13;p15), der(2)i(2)(p10)del(2)(p?13p24), der(3)t(3;17)(p21;q21), add(4)(q25), der(4)t(1;4)(q21;p15), add(6)(q25), add(8)(p12), der(10)add(10)(p13)add(10)(q21)(hsr), add(11)(p13), add(11)(q11), add(14)(q31), add(19)(q12); carries large hsr marker with genomic amplification of NMYC; resembles published karyotypes |
Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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