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| Cell line: | DT-40 |
| DSMZ no.: | ACC 636 |
| Species: | chicken (Gallus gallus) |
| Cell type: | B cell line |
| Origin: | established in 1985 from a bursal lymphoma, which was induced by infection of a 1 day old chicken with Rous-associated virus type 1 (RAV-1), an avian leukosis retrovirus carrying no cell-derived oncogene; cells are described in the literature to retain the ability to rearrange the immunoglobulin light chain gene (IgL) and to exhibit a high frequency of homologous gene targeting |
| Reference(s): | 14367, 14411, 14412, 15187, 15186 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| Depositor-specific restrictions: | Transfer to third parties needs to be negotiated with the depositor. |
| | DSMZ Cell Culture Data: |
| Morphology: | round cells growing in clumps of 10 to 100 cells in suspension; image; image; image |
| Medium: | 80% Advanced RPMI 1640 + 20% h.i. FBS + 1 mM sodium pyruvate + 2 mM L-glutamine + 4.5 g/L glucose + 50 µM mercaptoethanol |
| Subculture: | seed out at ca. 0.5 x 106 cells/ml; optimal split ratio of 1:2 to 1:3 every 2-3 days, maintain at 0.5-1.5 x 106 cells/ml |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 48 hours |
| Harvest: | maximum density at ca. 1.5 x 106 cells/ml |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in microbiological culture, PCR assays |
| Species validation: | Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Gallus gallus species. |
| Viruses: | SMRV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
