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| Cell line: | SH-2 |
| DSMZ no.: | ACC 643 |
| Species: | human (Homo sapiens) |
| Cell type: | acute myeloid leukemia |
| Origin: | established in 2005 from the bone marrow of a 35-year-old Chinese man with AML-M2 after idarubicin/cysteine arabinoside treatment; cells were described in the literature to express natural killer cell-related antigens in addition to myeloid ones and to exhibit TP53 gene alteration; cells are tumorigenic in nude and SCID mice |
| Reference(s): | 14865 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | round cells growing singly and partially in clumps in suspension; image; image; image |
| Medium: | 90% Iscove's MDM + 10% h.i. FBS |
| Subculture: | seed out at ca. 0.5 x 106 cells/ml; we recommend to start culture in a 12- or 24-well plate; optimal split ratio of 1:2 twice a week |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 80 hours |
| Harvest: | maximum densitiy of ca. 2 x 106 cells/ml |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in microbiological culture, PCR assays |
| Immunology: | CD3 -, CD4 +, CD13 +, CD14 -, CD15 +, CD19 -, CD33 +, CD34 -, CD56 +; image |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human hyperdiploid karyotype with 8% polyploidy; 57(54-58)<2n>X, -Y, +1, +2, +3, +4, +6, -11, +12, +13, +15, +18, +19, +22, +2mar, del(1)(q31.2q42)x2, der(4)t(1;4)(p22;q32), der(16)t(16;17)(q24;q12); related to published karyotype |
| Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
