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Cell line: | MDA-MB-453 |
DSMZ no.: | ACC 65 |
Species: | human (Homo sapiens) |
Cell type: | breast carcinoma |
Origin: | established from an pericardial fluid of a 48-year-old woman with invasive breast carcinoma in 1976; described as ER and PR negative cell line; listed in the CCLE (ref 18232); RNA sequence data are available (see Ref 40906 and RNA-Seq) |
Reference(s): | 14415, 14960, 18232, 18234, 40384, 40906 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | epithelial-like, round, loosely attached monolayers; image; image; image; image; image |
Medium: | 80-90% Leibovitz's L-15 medium + 10-20% h.i. FBS |
Subculture: | seed out at 2-4 x 106 cells/80 cm2; split confluent culture 1:2 to 1:6 using trypsin/EDTA; cells can partially be detached by tapping the flask; cells may need several days to re-adhere again |
Incubation: | at 37 °C without CO2 gas exchange (no filter lid, lid of flask closed) |
Doubling time: | ca. 50-60 hours |
Harvest: | about 25-35 x 106 cells/80 cm2 |
Storage: | frozen with 85% medium, 10% FBS, 5% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | initial contamination was eliminated with Ciprobay (ciprofloxacin), then negative in DAPI, microbiological culture, RNA hybridization, and continuously in PCR assays |
Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Cytogenetics: | human hypotetraploid karyotype with 6% polyploidy and near diploid sideline (2%) - 89(85-91)<4n>XXX, -X - highly rearranged leading to extensive marker formation, including der(5)t(5;?14)(p11;q11)x2 - dm present in 12% cells |
Molec. Genetics: | Western blot: ER and PR negative, while AR and HER2 positive |
Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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