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| Cell line: | WILL-2 |
| DSMZ no.: | ACC 652 |
| Species: | human (Homo sapiens) |
| Cell type: | B cell lymphoma |
| Origin: | established in 2008 post-mortem from the ascites of a 63-year-old woman with diffuse large B-cell lymphoma after relapse |
| Reference(s): | 14974 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| Depositor-specific restrictions: | Transfer to third parties needs to be negotiated with the depositor. |
| | DSMZ Cell Culture Data: |
| Morphology: | small and mostly single round cells growing in suspension; image; image; image |
| Medium: | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture: | seed out at ca. 1 x 106 cells/ml in a 24-well-plate; cells may grow slowly during the first days after thawing, but recover soon and grow fast in log-phase; optimal split ratio of 1:2 to 1:4 every 2-3 days; maintain at 1-2 x 106 cells/ml |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 36-48 hours |
| Harvest: | maximum densitiy of ca. 3 x 106 cells/ml |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in microbiological culture, PCR assays |
| Immunology: | CD3 -, CD10 +, CD13 -, CD19 +, CD20 (+), CD38 +, CD138 +, HLA-DR +; image |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human near-diploid karyotype; 46(43-47)<2n>XX, del(X)(q23), dup(1)(q21q32), ins(1;X)(q21;q27q23), t(4;17)(p15;q12). t(5;9)(q14;p21), t(8;22)(q24;q11), t(14;18)(q32;q21); carries t(8;22) rearrangement effecting IGL-MYC, and t(14;18) rearrangements effecting IGH-BCL2; resembles published karyotype |
| Molec. Genetics: | expression of fusion gene IGH-BCL2 confirmed by RT-PCR |
| Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
