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| Cell line: | NMB |
| DSMZ no.: | ACC 657 |
| Species: | human (Homo sapiens) |
| Cell type: | neuroblastoma |
| Origin: | established in 1974 from a bone marrow metastasis of a 10-month-old girl with neuroblastoma; cells are described in the literature to express an opiate binding site |
| Reference(s): | 14359, 14407, 15137 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| Depositor-specific restrictions: | Transfer to third parties needs to be negotiated with the depositor. |
| | DSMZ Cell Culture Data: |
| Morphology: | polymorphic cells (mostly epithelial with short neurites) growing adherently in monolayers; image; image; image |
| Medium: | 90% DMEM (4.5 g/L glucose) + 10% h.i. FBS |
| Subculture: | seed out at ca. 1-2 x 106 cells/80 cm2 flask; split confluent culture 1:4 to 1:10 once or twice a week using trypsin/EDTA |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 40-50 hours |
| Harvest: | cell harvest of ca. 40 x 106 cells/80 cm2 |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | initial contamination was eliminated with Baytril (enrofloxacin), then negative in microbiological culture and continuously in PCR assays |
| Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
