| | |
|---|
| Cell line: | LAN-2 |
| DSMZ no.: | ACC 671 |
| Species: | human (Homo sapiens) |
| Cell type: | neuroblastoma |
| Origin: | established in 1977 from the primary tumor of a 3-year-old girl with neuroblastoma (clinical Stage IV) |
| Reference(s): | 14949 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| Depositor-specific restrictions: | Transfer to third parties needs to be negotiated with the depositor. |
| | DSMZ Cell Culture Data: |
| Morphology: | adherent flat cells with processes accompanied by loosely adherent round cells; image; image |
| Medium: | 40% RPMI 1640 + 40% MEM (with Earle's salts) + 20% h.i. FBS + 2 mM L-glutamine + non-essential amino acids |
| Subculture: | seed out at 1-2 x 106 cells/80 cm2 flask; split confluent culture 1:2 to 1:4 every 4-6 days using trypsin/EDTA; (cells might grow slowly after thawing); washing of cells with PBS may be recommended to reduce occuring debris |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 50-70 hours |
| Harvest: | cell harvest of ca. 55 x 106 cells/175 cm2 |
| Storage: | frozen with 35% RPMI 1640, 35% MEM (with Earle's salts), 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in microbiological culture, PCR assays |
| Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
