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Cell line: | BC-3 |
DSMZ no.: | ACC 679 |
Species: | human (Homo sapiens) |
Cell type: | B cell lymphoma |
Origin: | established in 1995 from the pleural effusion of a 85-year-old man with B-NHL (primary effusion lymphoma, PEL) not infected with HIV; cells were shown to carry the human herpesvirus 8 (HHV-8), but not the Epstein-Barr virus (EBV). Exome and RNA sequence data are available (see Ref 18187 and
Exome sequence and RNA-Seq) |
Reference(s): | 15851, 18187 |
Biosafety level: | 2 |
Risk assessment: | The cell line carries HHV-8 sequences which were detected by PCR and Southern blot. We did not determine the infection mode of this herpes virus and cannot exclude the secretion of the viruses. The cell line is thus categorized biosafety level 2. |
Permissions and restrictions: | A, B |
| DSMZ Cell Culture Data: |
Morphology: | round cells growing singly and in clusters in suspension; image |
Medium: | 80% RPMI 1640 + 20% h.i. FBS |
Subculture: | seed out at ca. 0.5 x 106 cells/ml; maintain at ca. 0.2-1 x 106 cells/ml; split saturated culture 1:2 to 1:3 every 2-4 days |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | ca. 50-60 hours |
Harvest: | maximum densitiy of ca. 1.5 x 106 cells/ml |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in microbiological culture, PCR assays |
Immunology: | CD3 -, CD10 -, CD13 -, CD19 -, CD20 -, CD34 -, CD37 +, CD38 +, cyCD79a -, CD80 -, CD138 +, HLA-DR +, smIgG -, smIgM -, smkappa -, smlambda -; image |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Cytogenetics: | human flat moded hyperdiploid karyotype; 47(43-48)<2n>X, -Y, +7, +8, der(1)t(1;16)(q44;q13), del(7)(p21.1p21.2), t(8;15)(p21;q26), del(13)(q13), der(14)t(12;14)(q11;p11),t(16;22)(q13;q12), der(19)t(19;19)(p13.1;q13.3), del(20)(p11.2p11.3);
resembles published karyotype |
Viruses: | PCR: EBV -, HBV -, HCV -, HHV-8 +, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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