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| Cell line: | NALM-21 |
| DSMZ no.: | ACC 682 |
| Species: | human (Homo sapiens) |
| Cell type: | B cell precursor leukemia |
| Origin: | established from a bone marrow specimen of a 63-year-old man with acute undifferentiated leukemia (AUL) at relapse in 1990; cells were described in the literature to carry two Philadelphia chromosomes t(9;22) with a BCR-ABL1 e1-a2 fusion gene; the cell line is a serial sister cell line of NALM-20 (ACC 681) |
| Reference(s): | 15848, 18261 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | round cells growing singly and in clusters in suspension; image; image; image; image |
| Medium: | 80-90% RPMI 1640 + 10-20% h.i. FBS |
| Subculture: | seed out at ca. 0.8-1.0 x 106 cells/ml; maintain at ca. 0.5-2.0 x 106 cells/ml; split saturated culture 1:2 to 1:3 every 2-3 days |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 50 hours |
| Harvest: | maximum densitiy of ca. 3 x 106 cells/ml |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in microbiological culture, PCR assays |
| Immunology: | CD3 -, CD10 +, CD13 +, CD19 +, CD38 +, CD80 -, CD138 -, HLA-DR +, sm/cyIgG -, sm/cyIgM -, sm/cykappa -, sm/cylambda -; image |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human near-diploid karyotype; 46(44-48)<2n>X, -Y, +8, inv(4)(p14q35), i(8)(q10)x2, t(9;22)(q34;q11); carries t(9;22) effecting BCR-ABL1 rearrangement; resembles published karyotype |
| Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
