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| Cell line: | BCBL-1 |
| DSMZ no.: | ACC 683 |
| Species: | human (Homo sapiens) |
| Cell type: | B cell lymphoma |
| Origin: | established from the peritoneal effusion of a 40-year-old man with B-NHL (primary effusion lymphoma, PEL); cells were shown to carry the human herpesvirus type 8 (HHV-8), but not the Epstein-Barr virus (EBV). Exome and RNA sequence data are available (see Ref 18187 and
Exome sequence and RNA-Seq) |
| Reference(s): | 15854, 14483, 15200, 18187 |
| Biosafety level: | 2 |
| Risk assessment: | The cell line carries HHV-8 sequences which were detected by PCR and Southern blot. We did not determine the infection mode of this herpes virus and cannot exclude the secretion of the viruses. The cell line is thus categorized biosafety level 2. |
| Permissions and restrictions: | A, B |
| | DSMZ Cell Culture Data: |
| Morphology: | round/multiformed cells growing singly in suspension; image |
| Medium: | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture: | seed out at ca. 0.5 x 106 cells/ml; maintain at ca. 0.3-1.0 x 106 cells/ml; split saturated culture 1:2 to 1:4 every 2-4 days; immediately after thawing, viability might be rather low (60-70%) |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 50 hours |
| Harvest: | maximum densitiy of ca. 1 x 106 cells/ml |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in microbiological culture, PCR assays |
| Immunology: | CD3 -, CD10 -, CD13 -, CD19 -, CD20 -, CD34 -, CD38 +, cyCD79a -, CD80 -, CD138 +, HLA-DR +, sm/cyIgG -, sm/cyIgM -, sm/cykappa -, sm/cylambda -; image |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human highly rearranged flat-moded hypertetraploid karyotype with near-diploid and near-octaploid sidelines; 91-100<4n>XX, -Y, -Y, +2, +2, +6, +7, +7, +8, -9, -10, -11, +12, -13, -13, +15, -16, -22, -22, +2, -5mar, dup(1)(q12q44), del(1)(p11)dup(1)(q12q44), del(2)(p11)x1-2, der(2)t(2;5)(q35;?q32)t(5;8)(?q35;q22), der(4)t(4;8)(q24;q24), t(6;17)(p23;q21), del(9)(q12q21), der(11)t(11;15)(q23;q21), der(11)t(11;15)(q25;q25), der(13)t(13;13)(p12;q21)x1-2, der(14)t(4;14)(q31;q32)x2, der(14)t(14;22)(p12;q12)t(4;14)(q31;q22)x2, der(15)t(8;15)(q21;q25); carries IGH rearrangements and genomic MYC amplification; related to published karyotype |
| Viruses: | PCR: EBV -, HBV -, HCV -, HHV-8 +, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
