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Cell line: | MINO |
DSMZ no.: | ACC 687 |
Species: | human (Homo sapiens) |
Cell type: | B cell lymphoma |
Origin: | established from peripheral blood mononuclear cells of a 68-year-old woman with mantle cell lymphoma at relapse in 1997; described in the literature to carry a t(11;14)(q13;q32) leading to CCND1-IGH (BCL1-IGH) fusion gene. Exome and RNA sequence data are available (see Ref 18187 and
Exome sequence and RNA-Seq) |
Reference(s): | 15849, 15850, 18187 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
Depositor-specific restrictions: | Transfer to third parties needs to be negotiated with the depositor. |
| DSMZ Cell Culture Data: |
Morphology: | round cells growing singly in suspension; image; image |
Medium: | 80% RPMI 1640 + 20% h.i. FBS |
Subculture: | seed out at ca. 1.0 x 106 cells/ml; maintain at ca. 0.5-1.0 x 106 cells/ml; split culture 1:2 to 1:3 every 2-3 days |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | ca. 50 hours |
Harvest: | maximum density at ca. 1.5 x 106 cells/ml |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in microbiological culture, PCR assays |
Immunology: | CD3 -, CD10 -, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, CD138 +, HLA-DR +, cyIgG -, cyIgM +, cykappa -, cylambda +; image |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Cytogenetics: | human flat moded hypertriploid karyotype; 70-75<3n>XXY, -3, -5, +7, +14, -16, -16, -18, -19, +20, +20, -21, +4mar, del(3)(q13)x1-2, del(6)(22), i(7)(p10), der(8)t(8;14)(q24;q32)t(14;11)(q32;q13)x2, t(8;14)(q24;q32)x2, der(11)t(11;14)(q2?;q11)t(14;8)(q32;q24)x1-2, der(11)t(11;14)(q13;q32)x2, add(14)(q21), der(13)t(3;13)(q25;q32)x1-2; carries complex rearrangements of IGH with MYC and CCND1 (BCL1) consistent with MCL; although presence of Y-chromosome is inconsistent with published patient gender, karyotype otherwise resembles that published |
Molec. Genetics: | expression of fusion gene IGH-CCND1 confirmed by RT-PCR |
Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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