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| Cell line: | ELF-153 |
| DSMZ no.: | ACC 693 |
| Species: | human (Homo sapiens) |
| Cell type: | acute myeloid leukemia |
| Origin: | established 1988 from a 41-year-old man with acute myeloid leukemia (AML-M7) during refractory relapse initially presenting with acute myelofibrosis. Exome and RNA sequence data are available (see Ref 18187 and
Exome sequence and RNA-Seq) |
| Reference(s): | 16345, 16346, 18187 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | round cells growing singly in suspension; occasionally semi adherent cells and a few giant cells are present; image; image; image |
| Medium: | 80% RPMI 1640 + 20% h.i. FBS + 5 ng/ml GM-CSF |
| Subculture: | seed out at ca. 0.8 x 106 cells/ml in 24-well plates; maintain at ca. 0.8-3 x 106 cells/ml; split culture 1:3 to 1:8 every 3-5 days. CAVEAT: by favoring selective growth, suboptimal culture of cytokine-dependent cell lines may promote outgrowth of factor-independent subclones. Selective conditions include cytokine insufficiency and inadequate cell density. Thus, the DSMZ cannot guarantee indefinite stability of factor-dependence of cell lines. |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 40-60 hours |
| Harvest: | maximum density at ca. 5 x 106 cells/ml |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in microbiological culture, PCR assays |
| Immunology: | CD3-, CD4+, CD13+, CD14-, CD15-, CD19, CD33+, CD34+, HLA-DR+, cyCD3-, cyCD68-; image |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human hypodiploid karyotype with 8% polyploidy; 42(40-43)<2n>X, -Y, -7, -14, -17, del(5)(q13q32), der(6)t(6;7)(p23;p11), i(7)(q10), der(12)t(12;14)(p12;q11), add(20)(q13)x1-2; matches published karyotype |
| Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
