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Cell line: | OCI-LY18 |
DSMZ no.: | ACC 699 |
Species: | human (Homo sapiens) |
Cell type: | B cell lymphoma |
Origin: | established in 1988 from the pleural fluid of a 56-year-old man with diffuse large B cell lymphoma (DLBCL, stage IIIB, at relapse); cells were described in the literature to be EBV negative and to carry a three-way translocation t(8;18;14) leading to alterations of MYC, BCL2, and IGH genes |
Reference(s): | 14427, 16050, 16051 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
Depositor-specific restrictions: | Transfer to third parties needs to be negotiated with the depositor. |
| DSMZ Cell Culture Data: |
Morphology: | small round cells growing singly in suspension; image; image; image |
Medium: | 90% RPMI 1640 + 10% h.i. FBS |
Subculture: | seed out at ca. 0.5 x 106 cells/ml; maintain at ca. 0.5-3.0 x 106 cells/ml; split culture 1:3-1:5 every 2-3 days |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | ca. 30-40 hours |
Harvest: | maximum density at ca. 3.5 x 106 cells/ml |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in microbiological culture, PCR assays |
Immunology: | CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD34 -, CD38 +, cyCD79a +, CD138 +, HLA-DR +, sm/cyIgG -, sm/cyIgM +, sm/cykappa -, sm/cylambda +; image |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Cytogenetics: | human hyperdiploid karyotype with 4% polyploidy; 50(48-51)<2n>X, -Y, +9, +17, +18, +18, +18, der(8)t(8;14)(q24;q32)t(14;18)(q32;q21), del(9)(q21.1), dup(12)(q11q15), der(14)t(14;18)(q32;q21), der(17)t(17;18)(p11.2;q21)t(18;14)(q21;q32), der(18)t(14;18)(q32;q21)x2, der(18)t(14;18)(q32;q21)t(14;8)(q32;q24)x2; carries complex series of two break IGH rearrangements involving MYC and BCL2 on ders(8)/(14)/(17)/(18); resembles published karyotype |
Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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