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| Cell line: | KASUMI-3 |
| DSMZ no.: | ACC 714 |
| Species: | human (Homo sapiens) |
| Cell type: | acute myeloid leukemia |
| Origin: | established from blast cells of the bone marrow of a 57-year-old man with minimally differentiated acute myeloblasic leukemia (AML M0); cells were described in the literature to carry a t(3;7) leading to alteration of MECOM-TRB (EVI1-TRB) genes and to MECOM (EVI1) overexpression |
| Reference(s): | 16469 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | round to polymorphic cells growing singly and in small clumps in suspension; image; image; image |
| Medium: | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture: | start with ca. 0.5-1 x 106 cells/ml in 24-well plates; later, maintain at ca. 0.3-1 x 106 cells/ml in flasks; split saturated culture 1:2 every 3-4 days |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 3-4 days |
| Harvest: | maximum density at ca. 1.5 x 106 cells/ml |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in microbiological culture, PCR assays |
| Immunology: | CD2 -, cy/smCD3 -, CD4 +, CD5 -, CD7 +, CD8 -, CD13 +, CD14 -, CD15 -, CD19 -, CD20 -, CD33 +, CD34 +, cyCD68 +, HLA-DR +; image |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human pseudodiploid karyotype with 9% polyploidy; 46(43-47)<2n>XY, der(2)t(2;12)(p15;p13), der(3)t(3;7)(q26;q35)t(7;8)(q22;q23), der(4)t(4;8)(q13;q21), del(5)(q15), der(5)t(5;10)(q33;q24), der(7)t(3;7)(q26;q35)del(7)(p1?), der(8)t(4;8)(q13;q12), del(9)(q32), del(10)(q24), add(12)(p11), add(16)(q13.2) ; carries rearrangement of MECOM (EVI1) with TRB (TCRB); resembles published karyotypes |
| Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
