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Cell line: | MAVER-1 |
DSMZ no.: | ACC 717 |
Species: | human (Homo sapiens) |
Cell type: | B cell lymphoma |
Origin: | established from the peripheral blood of a 82-year-old man with B-cell non-Hodgkin lymphoma (B-NHL; mantle cell lymphoma) in 2003; described in the literature to carry the t(11;14) affecting the CCND1 (BCL1) and IGH genes |
Reference(s): | 16453 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
Depositor-specific restrictions: | Transfer to third parties needs to be negotiated with the depositor. |
| DSMZ Cell Culture Data: |
Morphology: | round cells growing singly or in small clumps in suspension; image; image; image;
image; |
Medium: | 80-90% RPMI 1640 + 10-20% h.i. FBS |
Subculture: | seed out at ca. 1 x 106 cells/ml; maintain at ca. 0.5-1.5 x 106 cells/ml; split culture 1:2 every 2-3 days; we recommend to start the culture with 20% FBS |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | ca. 48 hours |
Harvest: | maximum density at ca. 2 x 106 cells/ml |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in microbiological culture, PCR assays |
Immunology: | CD3 -, CD10 -, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, cyCD79a +, HLA-DR +, sm/cyIgG -, sm/cyIgM +, sm/cykappa -, sm/cylambda +; image |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Cytogenetics: | human highly rearranged extreme hypodiploid karyotype with 10% polyploidy; 40(34-42)<2n>XY, -8, -12, -13, -14, -17, -22, der(1)dup(1)(p34p36)t(1;3)(p36;q21), der(3)del(3)(p13)t(3;14)(q27;q12), der(4)t(4;8)(q35;q24), der(6)t(6;14)(q13;q11)t(14;11)(q32;q13), der(8)dup(8)(q22q24)ins(8;7)(q24;?), ider(9)(q10)t(8;9)(q24;q34-35)t(8;22)(q24;q11), ider(9)del(9)(p21), der(10)t(8;10)(q13;p12), der(11)t(11;14)(q13;q32), der(12)t(12;12)(p13;q24)?del(12)(p11q11), der(14)t(3;14)(q13;q12), der(15)t(11;15)(p13;p11)dup(15)(q11q25), der(21)t(13;21)(q31;p11), der(22)t(18;22)(q22q23)dup(18)(q22q23)t(18;11)(q23;p13); resembles published karyotype; carries t(11;14) effecting rearrangement of IGH with CCND1 together with non-IGH rearrangements of MYC and amplification of IGH/CCND1 and BCL2 regions; karyotypically related but distinct sidelines present |
Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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