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| Cell line: | L-540 |
| DSMZ no.: | ACC 72 |
| Species: | human (Homo sapiens) |
| Cell type: | Hodgkin lymphoma |
| Origin: | established from the bone marrow of a 20-year-old woman with Hodgkin lymphoma (nodular sclerosis; stage IVB, pre-terminal stage) |
| Reference(s): | 14462, 14463, 15213, 14478, 40211 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | round to polymorph cells growing singly in suspension, heterogeneity in cell size; image; image; image; image; image |
| Medium: | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture: | seed out at ca. 0.5 x 106 cells/ml; after thawing culture first in 24-well-plates; cells do not grow during the first 2-3 days after thawing and viability may drop to 50-60%; in logarithmic growth split saturated culture 1:2 every 3 days, exchange spent medium every 1-2 days |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 70 hours |
| Harvest: | saturation density at about 1.5 x 106 cells/ml |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | initial contamination was eliminated with BM-Cyclin (tiamulin & minocycline), then negative in DAPI, microbiological culture, RNA hybridization, and continuously in PCR assays |
| Immunology: | CD2+, CD3-, CD13-, CD14., CD15(+), CD19-, CD21-, CD25(+), CD30+, CD34-; image |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human flat-moded hypotriploid karyotype with 16% polyploidy - 59-64<3n>XX, -X, -1, +2, -6, +7, -8, -9, -10, -11, -13, -14, -16, -17, -18, +22, +5-6mar, der(1;5)(q10;p10)x1-2, der(2;12)(q10;q10), der(2)t(2;?8)(q3?7;q2?1)x1-2, ins(5;?)(p11;?), add(7)(p1?), add(8)(q24)x1-2, add(9)(p1?), add(11)(q24), der(15)t(1;15)(p21;p13)x2, add(19)(p11), add(21)(p13) |
| Molec. Genetics: | expression of fusion gene TNK1-TMEM256 (TNK1-C17orf61) confirmed by RT-PCR |
| Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | Growing culture
(please inquire for exact delivery time) | | 1040,- € | | Frozen culture | | 520,- € | | DNA isolated from cell line (25 µg) | | 650,- € | | DNA isolated from cell line (5 µg) | | 160,- € |
see price list |
| | Print data sheet |
