| | |
|---|
| Cell line: | TK-6 |
| DSMZ no.: | ACC 723 |
| Species: | human (Homo sapiens) |
| Cell type: | chronic myeloid leukemia |
| Origin: | established 1995 from the pleural effusion of a 30-year-old man with chronic myelogenous leukemia (CML) in T cell lineage blast crisis after bone marrow transplantation; cells carry the Philadelphia chromosome with a e14-a2 (b3-a2) BCR-ABL1 fusion gene. Exome and RNA sequence data are available (see Ref 18187 and
Exome sequence and RNA-Seq) |
| Reference(s): | 16449, 16450, 14484, 18187 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| Depositor-specific restrictions: | Transfer to third parties needs to be negotiated with the depositor. |
| | DSMZ Cell Culture Data: |
| Morphology: | round cells growing singly in suspension; image; image; image; image |
| Medium: | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture: | seed out at ca. 0.5 x 106 cells/ml; split saturated culture 1:2 every 3 days |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 60-72 hours |
| Harvest: | maximum density at ca. 2.8 x 106 cells/ml |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in microbiological culture, PCR assays |
| Immunology: | CD2 -, CD3 -, CD5 +, CD6 +, CD7 +, CD10 -, CD13 -, CD14 -, CD19 -, CD20 -, CD34 -, cyCD68 -, TCRalpha/beta -, TCRgamma/delta -, HLA-DR -;
image |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human hyperdiploid karyotype; 48(46-50)<2n>XY, +22, +22, del(1)(q24), del(6)(q21q24), dup(7)(p1?4q3?5)inv(7)(p14q35), t(9;22)(q34;q11), add(21)(p11), der(22)?inv(22)(p11q11)t(9;22)(q34;q11)x2; carries complex t(9;22) effecting BCR-ABL1 rearrangement with 3x genomic amplification in der(22); matches published karyotype |
| Molec. Genetics: | expression of fusion gene BCR-ABL1 e14-a2 (b3-a2) confirmed by RT-PCR |
| Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |
| Supplied as: | | Delivery form | | Prices | | Growing culture
(please inquire for exact delivery time) | | 1040,- € | | Frozen culture | | 520,- € | | DNA isolated from cell line (25 µg) | | 650,- € | | DNA isolated from cell line (5 µg) | | 160,- € |
see price list |
| | Print data sheet |
