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| Cell line: | OCI-AML1 |
| DSMZ no.: | ACC 726 |
| Species: | human (Homo sapiens) |
| Cell type: | acute myeloid leukemia |
| Origin: | established in 1987 from the peripheral blood of a 73-year-old woman with acute myelomonocytic leukemia (AML M4) |
| Reference(s): | 15050, 15051, 16751, 16805, 14987 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| Depositor-specific restrictions: | Transfer to third parties needs to be negotiated with the depositor. |
| | DSMZ Cell Culture Data: |
| Morphology: | round cells growing singly in suspension; image; image; image; image |
| Medium: | 60% alpha-MEM (with ribo- and deoxyribonucleosides) + 20% h.i. FBS + 20% vol conditioned medium of cell line 5637 (DSM ACC 35) (or 10-20 ng/ml GM-CSF) |
| Subculture: | seed out at ca. 0.8-1.0 x 106 cells/ml; maintain at ca. 0.5-1.0 x 106 cells/ml; split saturated culture 1:2 every 3-4 days; possibly no cell growth during first week after thawing; we recommend to culture the cells in 24-well plates; CAVEAT: by favoring selective growth, suboptimal culture of cytokine-dependent cell lines may promote outgrowth of factor-independent subclones. Selective conditions include cytokine insufficiency and inadequate cell density. Thus the DSMZ cannot guarantee indefinite stability of factor-dependence of cell lines. |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 3-4 days |
| Harvest: | maximum density at ca. 2 x 106 cells/ml |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in microbiological culture, PCR assays |
| Immunology: | CD3-, CD4(+), CD13+, CD14-, CD15+, CD19-, CD33-, CD34+, HLA-DR+, cyCD68+; image |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human diploid karyotype; 46(41-46)<2n>XX; no consistent abnormality detected; lacks t(6;8) present in sister cell line OCI-AML1a |
| Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
