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Cell line: | HT-93 |
DSMZ no.: | ACC 735 |
Species: | human (Homo sapiens) |
Cell type: | acute myeloid leukemia |
Origin: | established in 1993 from the peripheral blood of a 66-year-old man with acute promyelocytic leukemia (AML FAB M3) at relapse; described in the literature to carry t(15;17) and t(1;12) leading to PML-RARA and ETV6-ABL2 (ETV6-ARG) fusion genes, respectively |
Reference(s): | 17286, 17287, 17298, 17299 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
Depositor-specific restrictions: | Transfer to third parties needs to be negotiated with the depositor. |
| DSMZ Cell Culture Data: |
Morphology: | single cells growing in suspension; image; image |
Medium: | 80% RPMI 1640 + 20% h.i. FBS |
Subculture: | seed out and maintain at ca. 0.5-1.0 x 106 cells/ml; optimal split ratio 1:2 every 2-3 days; culture should be started with 20% FBS in 24-well-plates |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | ca. 50 hours |
Harvest: | maximum density at ca. 1.5 x 106 cells/ml |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative PCR assay |
Immunology: | CD3 -, CD19 -, CD33 +, CD34 +, cyCD68 +, HLA-DR -; image |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Cytogenetics: | human near-diploid karyotype; 46(44-46)<2n>XY, t(1;12)(q25;p13), der(2)t(1;2)(q25;q31), der(4)t(1;4)(q21;q25)t(1;12)(q25;p13), der(6)del(6)(p23)t(4;6)(q25;q23), t(15;17)(q22;q21); carries complex t(1;12) and t(15;17) rearrangements effecting ETV6-ABL2 (ETV6-ARG) and PML-RARA rearrangements, respectively; resembles published karyotyp |
Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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