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Cell line: | 17.94 |
DSMZ no.: | ACC 741 |
Species: | human (Homo sapiens) |
Cell type: | Wilms´ tumor |
Origin: | established from the nephrectomy specimen of a large lobulated Wilms´ tumor of a 4-year-old girl; cells were described in the literature to express NCAM, SALL1, and CITED1, and to contain a TP53 mutation |
Reference(s): | 18006 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
Depositor-specific restrictions: | Transfer to third parties needs to be negotiated with the depositor. |
| DSMZ Cell Culture Data: |
Morphology: | pleomorphic epithelioid cells growing adherently in monolayers; the cytoplasm of the cells exhibits numerous granules; image; image; image; image; image |
Medium: | 80% DMEM (with 4.5 g/l glucose) + 20% h.i. FBS |
Subculture: | seed out at ca. 0.5-1 x 106 cells/25 cm2 flask; cells grow slowly and may need 1-2 days for adherence to the plastic surface;
split confluent culture 1:2 once a week using trypsin/EDTA (wash cells twice with PBS before adding trypsin/EDTA) |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | ca. 1 week |
Harvest: | cell harvest of ca. 10 x 106 cells/80 cm2 |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | contamination was eliminated with BM-Cyclin (tiamulin & minocycline), then negative in PCR assay |
Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV + |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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