| | |
|---|
| Cell line: | PACADD-159 |
| DSMZ no.: | ACC 745 |
| Species: | human (Homo sapiens) |
| Cell type: | pancreas carcinoma |
| Origin: | established in 2012 from a primary pancreatic tumor of a 78-year-old man with ductal adenocarcinoma located at the tail of the pancreas (intermediate grade G2, pT3N1M0) |
| Reference(s): | 17618 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| Depositor-specific restrictions: | Transfer to third parties needs to be negotiated with the depositor. |
| | DSMZ Cell Culture Data: |
| Morphology: | epithelioid cells growing adherently in monolayers |
| Medium: | 80% mixture of DMEM and Keratinocyte SFM (at 1:1) + 20% h.i. FBS |
| Subculture: | seed out at ca. 1 x 106 cells/25 cm2 flask; split confluent culture 1:2 once a week using trypsin/EDTA (wash cells twice with PBS before adding trypsin/EDTA and apply trypsin/EDTA for up to 20 min. to detach cells); cells may take 1-2 days to adhere again |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 1 week |
| Harvest: | cell harvest of ca. 5 x 106 cells/80 cm2 |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in PCR assay |
| Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
