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| Cell line: | U-CH2 |
| DSMZ no.: | ACC 749 |
| Species: | human (Homo sapiens) |
| Cell type: | chordoma |
| Origin: | established in 2000 from the malignant sacral chordoma of a 72-year-old woman; cell line is described in the literature having a complex karyotype with dmins and manifold chromosomal abberrations |
| Reference(s): | 17941 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| Depositor-specific restrictions: | Transfer to third parties needs to be negotiated with the depositor. |
| | DSMZ Cell Culture Data: |
| Morphology: | typical physalipherous chordoma phenotype growing strictly adherent |
| Medium: | 80% mixture of Iscove's MDM and RPMI 1640 (at 4:1) + 20% FBS (do not heat-inactivate!) + 2 mM glutamine |
| Subculture: | seed out at ca. 0.5 x 106 cells/80 cm2 flask; split confluent culture every 6-8 days using trypsin/EDTA or TrypLE Express |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 5-7 days |
| Harvest: | cell harvest of ca. 3 x 106 cells/80 cm2 |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in PCR assay |
| Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
