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Cell line: | BJAB |
DSMZ no.: | ACC 757 |
Species: | human (Homo sapiens) |
Cell type: | Burkitt lymphoma |
Origin: | established in 1973 from the inguinal tumor of an EBV-negative 5-year-old girl with Burkitt lymphoma at the preterminal stage; although the tumor tissue was experimentally exposed to EBV, neither EBV sequences nor EBV antigens were detectable in the established cell line. Exome and RNA sequence data are available (see Ref 18187 and
Exome sequence
and RNA-Seq) |
Reference(s): | 18899, 15230, 18187 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | round to polymorphic cells growing in suspension singly cells and in clumps; image; image |
Medium: | 80% RPMI 1640 + 20% h.i. FBS |
Subculture: | seed out at ca. 0.5-0.7 x 106 cells/ml; optimal split ratio 1:2 -1:5 every 2-3 days; culture flask should be kept horizontal |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | ca. 30-40 hours |
Harvest: | maximum density at ca. 1.5 x 106 cells/ml |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in PCR assay |
Immunology: | CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, HLA-DR +, sm/cyIgG -, sm/cyIgM +, sm/cykappa +, sm/cylambda -; image |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Cytogenetics: | human hypodiploid karyotype; 44(40-46)<2n>XX, -10, -21, der(6)t(6;10)(p22;p12), der(7)t(1;7)(p36;p22), t(11;17)(q24;q24), der(11)t(11;17)(q24;q24)t(11;6)(p15;q11)t(6;5)(q22;?), ider(13)(q10)t(3;13)(q26;q14), der(13)t(10;13)(q11;p11)dup(13)(q11q32)t(13;21)(q32;q22), der(21)t(13;21); does not correspond with previous BJA-B karyotypes published; no IG/MYC translocation detected |
Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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