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Cell line: | LS-174T |
DSMZ no.: | ACC 759 |
Species: | human (Homo sapiens) |
Cell type: | colon adenocarcinoma |
Origin: | established in 1974 from the moderately well-differentiated primary colonic adenocarcinoma (Duke´s type B) of a 58-year-old woman; cells were described in the literature to produce large amounts of carcinoembryonic antigen (CEA) and to express C-Myc, N-Myc, H-Ras, and N-Ras |
Reference(s): | 18716 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
Depositor-specific restrictions: | Transfer to third parties needs to be negotiated with the depositor. |
| DSMZ Cell Culture Data: |
Morphology: | epithelial-like cells growing adherently as monolayer in tight clusters or islands; image; image; image; image |
Medium: | 90% RPMI 1640 + 10% h.i. FBS |
Subculture: | seed out at ca. 2-4 x 106 cells/80 cm2 flask; split confluent culture 1:5 - 1:10 every 3-5 days using trypsin/EDTA |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | ca. 30-40 hours |
Harvest: | cell harvest of ca. 20-25 x 106 cells/80 cm2 |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in PCR assay |
Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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