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| Cell line: | WA-C3CD5+ |
| DSMZ no.: | ACC 769 |
| Species: | human (Homo sapiens) |
| Cell type: | chronic B cell leukemia |
| Origin: | established in 1994 from the peripheral blood leukocytes of a man with B-type chronic lymphocytic leukemia (CLL, Rai stage I at diagnosis); CD5 positive sister cell line of WA-OSEL (DSM ACC 767) |
| Reference(s): | 19334, 19172 |
| Biosafety level: | 2 |
| Risk assessment: | The cell line is positive for HHV-4 (EBV) by PCR analysis. Additionally, expression of immediate-early protein BZLF-1 was also positive by western blot in untreated and phorbol ester / sodium butyrate stimulated cells. The infection was classified as lytic associated with a production of active viruses. A transmission of EBV during handling of the cells is possible and the cell line is thus categorized biosafety level 2. |
| Permissions and restrictions: | A, B |
| Depositor-specific restrictions: | Transfer to third parties needs to be negotiated with the depositor. |
| | DSMZ Cell Culture Data: |
| Morphology: | round cells growing singly and in small clusters in suspension |
| Medium: | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture: | seed out at ca. 1 x 106 cells/ml in 24-well plates; optimal split ratio 1:2 - 1:3 every two days; maintain at ca. 0.5 x 106 cells/ml |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 40 - 50 hours |
| Harvest: | maximum density at ca. 0.7-1 x 106 cells/ml |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in PCR assay |
| Immunology: | CD3 -, CD5 +, CD10 -, CD13 -, CD14 -, CD15 -, CD19 +, CD20 +, CD34 -, CD37 +, cyCD79a +, CD80 +, HLA-DR +, sm/cyIgG -, sm/cyIgM +, sm/cykappa +, sm/cylambda -; image |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human flat-moded hyperdiploid karyotype with 6% polyploidy;43-51<2n>XY, +9, +13, +21, der(6)t(6;9)(q14;q21), der(7)t(7;17)(q10;q10), del(13)(q14q21)x2, del(13)(q14.2q14.3);
nullisomy for DLEU2 locus via focal chr. 13 deletion associated with CLL; marginally related to published karyotype |
| Viruses: | PCR: EBV +, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
