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| Cell line: | UOC-M1 |
| DSMZ no.: | ACC 775 |
| Species: | human (Homo sapiens) |
| Cell type: | acute myeloid leukemia |
| Origin: | established at diagnosis in 1992 from the malignant bone marrow cells of a 68-year-old man with undifferentiated acute myeloid leukemia (FAB AML M1) of the megakaryoblastic lineage; cells are described in the literature to carry four copies of the KMT2A (MLL) gene |
| Reference(s): | 19887 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| Depositor-specific restrictions: | Transfer to third parties needs to be negotiated with the depositor. |
| | DSMZ Cell Culture Data: |
| Morphology: | round to elongated cells growing singly in suspension and some cells adherently; image |
| Medium: | 90% McCoy´s 5a + 10% h.i. FBS |
| Subculture: | seed out at ca. 0.5 x 106 cells/ml; optimal split ratio 1:2 every 2-3 days; maintain at ca. 0.5-1.5 x 106 cells/ml; adherent cells can be detached by tapping or using trypsin/EDTA |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 50-60 hours |
| Harvest: | maximum density at ca. 1.5-2 x 106 cells/ml |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in PCR assay |
| Immunology: | CD3 -, CD4 -, CD7 +, CD13 +, CD14 -, CD15 -, CD19 -, CD24 +, CD33 +, CD34 +, CD38 +, CD41 +, CD42 -, cyCD68 +, HLA-DR +; image |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human flat-moded hypodiploid karyotype; -39-44<2n>XY, -7, -9, -11, -14, -19, +21, +21, der(Y)t(Y;9)(p11;p13)t(9;Y)(p24;p11), del(5)(q14q35), der(5)t(Y;5)(q11;p15)t(Y;9)(q12;q11), der(9;11)(11qter->q11::19p1?->p1?::9pter->q11::19p1?->p1?::11q11->q25::11q14->qter), der(17)t(7;17)(p15;p13), r(19)(p13q11), der(21)t(16;21)(p11;p11), der(21t(19;21)(p11;p11), der(21)trp(21)(q21.2q22.3)t(11;21)(q22;q22.3); copy number increase (to four per diploid genome) of unrearranged KMT2A (MLL) locus; resembles published karyotype |
| Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
