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| Cell line: | JVE-015 |
| DSMZ no.: | ACC 786 |
| Species: | human (Homo sapiens) |
| Cell type: | colon carcinoma |
| Origin: | established from the tissue of a primary malignant tumor from the colon sigmoideum of a 66-year old woman; cells were described in the literature to carry mutations in the TP53, K-RAS, APC, and PIK3CA genes |
| Reference(s): | 18128 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| Depositor-specific restrictions: | Transfer to third parties needs to be negotiated with the depositor. |
| | DSMZ Cell Culture Data: |
| Morphology: | epitheloid cells growing adherently as monolayer and in large colonies |
| Medium: | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture: | seed out at ca. 1 x 106 cells/80 cm2 flask; split confluent culture 1:3 - 1:5 once or twice a week using trypsin/EDTA (wash twice with PBS and incubate with trypsin/EDTA for at least 15 min.) |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 3-4 days |
| Harvest: | cell harvest of ca. 10-15 x 106 cells/175 cm2 |
| Storage: | 70% RPMI 1640 + 20% FBS + 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in PCR assay |
| Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV + |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
