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| Cell line: | ETCC-007 |
| DSMZ no.: | ACC 791 |
| Species: | human (Homo sapiens) |
| Cell type: | breast ductal carcinoma |
| Origin: | established in 2010 from the tumor tissue biopsy of a 47-year-old woman with breast ductal carcinoma in situ (DCIS); cells were immortalized by stable transformation with human telomerase reverse transcriptase (hTERT); the ETCC-007 cell line is one of a series of five immortalized colonies from the same tumor sample (ETCC-006 (DSMZ ACC 790), ETCC-007, ETCC-008, ETCC-010, ETCC-011);
RNA sequence data are available (see Ref 40906 and RNA-Seq) |
| Reference(s): | 18123, 40906 |
| Biosafety level: | 1, GMO-S1 |
| Risk assessment: | The cell line ETCC-007 was established by stable lipofectamin transformation of breast carcinoma cells with the pGRN145 plasmid coding for the catalytic subunit of the human telomerase reverse transcriptase (hTERT) and for hygromycin resistence. Donor cells, vector und recipient cells match risik group 1 |
| Permissions and restrictions: | A, D |
| Depositor-specific restrictions: | Transfer to third parties needs to be negotiated with the depositor. |
| | DSMZ Cell Culture Data: |
| Morphology: | epithelioid cells growing adherently as monolayer; image; image |
| Medium: | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture: | seed out at ca. 1 x 106 cells/80 cm2 flask; split confluent culture 1:10 once or twice a week using trypsin/EDTA |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 40 hours |
| Harvest: | cell harvest of ca. 10 x 106 cells/80 cm2 |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in PCR assay |
| Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database; identical to ETCC-006 (DSMZ ACC 790) |
| Molec. Genetics: | triple-negative due to absence of ER, PR and weak HER2 expression in Western blots |
| Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV + |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
