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| Cell line: | ETCC-010 |
| DSMZ no.: | ACC 793 |
| Species: | human (Homo sapiens) |
| Cell type: | breast ductal carcinoma |
| Origin: | established in 2010 from the tumor tissue biopsy of a 47-year-old woman with breast ductal carcinoma in situ (DCIS); cells were immortalized by stable transformation with human telomerase reverse transcriptase (hTERT); the ETCC-010 cell line is one of a series of five immortalized colonies (ETCC-006, ETCC-007, ETCC-008, ETCC-010, ETCC-011) from the same tumor sample |
| Reference(s): | 18123 |
| Biosafety level: | 1, GMO-S1 |
| Risk assessment: | The cell line ETCC-010 was established by stable lipofectamin transformation of breast carcinoma cells with the pGRN145 plasmid coding for the catalytic subunit of the human telomerase reverse transcriptase (hTERT) and for hygromycin resistence. Donor cells, vector und recipient cells match risik group 1 |
| Permissions and restrictions: | A, D |
| Depositor-specific restrictions: | Transfer to third parties needs to be negotiated with the depositor. |
| | DSMZ Cell Culture Data: |
| Morphology: | epithelioid cells growing adherently as monolayer; image; image |
| Medium: | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture: | seed out at ca. 1 x 106 cells/175 cm2 flask; split confluent culture 1:10 - 1:20 once a week using trypsin/EDTA |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 30 hours |
| Harvest: | cell harvest of ca. 20 x 106 cells/175 cm2 |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in PCR assay |
| Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
