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| Cell line: | PCL-12 |
| DSMZ no.: | ACC 794 |
| Species: | human (Homo sapiens) |
| Cell type: | chronic B cell leukemia |
| Origin: | established in 2012 from the peripheral blood of a 73 year-old man with chronic lymphocytic leukemia (CLL Rai stage I, Binet stage A) after multiple chemotherapeutic treatments; cells are described in the literature to highly express CD19 and CD5; however, DSMZ immunoprofiling showed that the level of CD5 expressing cells decrease to about 10% after long-term cultivation |
| Reference(s): | 18125 |
| Biosafety level: | 2 |
| Risk assessment: | The cell line is positive for EBV (HHV-4) by PCR analysis. Additionally, expression of immediate-early protein BZLF-1 and lately expressed capsid protein were also positive by western blot in phorbol ester / sodium butyrate stimulated cells. The infection was classified as lytic associated with a at least inducible production of active viruses. A transmission of EBV during handling of the cells is possible and the cell line is thus categorized biosafety level 2. |
| Permissions and restrictions: | A, B |
| Depositor-specific restrictions: | Transfer to third parties needs to be negotiated with the depositor. |
| | DSMZ Cell Culture Data: |
| Morphology: | round cells growing in big clusters in suspension |
| Medium: | 80-90% RPMI 1640 + 10-20% h.i. FBS |
| Subculture: | seed out at ca. 0.5 x 106 cells/ml; optimal split ratio 1:2 - 1:3 every 2-3 days |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 40-50 hours |
| Harvest: | maximum density at ca. 1.5 x 106 cells/ml |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in PCR assay |
| Immunology: | CD3 -, CD 10 -, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, CD80 +, HLA-DR +; image |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Viruses: | PCR: EBV +, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
