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Cell line: | MBU-IM |
DSMZ no.: | ACC 796 |
Species: | human (Homo sapiens) |
Cell type: | keratinocytes |
Origin: | established in 2011 from primary keratinocytes of a healthy neonatal foreskin; cells were immortalized by transduction with retroviral vectors containing human telomerase and SV40 early region |
Reference(s): | 25648 |
Biosafety level: | 1, GMO-S1 |
Risk assessment: | Primary keratinocyte cells isolated from a neonatal foreskin were transduced with replication deficient amphotropic retroviruses containing a) the catalytic subunit of human telomerase reverse transcriptase hTERT, and b) SV40 early region for immortalization; donor, vector, and recipient cells are categorized to biosafety level 1 |
Permissions and restrictions: | A, D |
Depositor-specific restrictions: | Transfer to third parties needs to be negotiated with the depositor. |
| DSMZ Cell Culture Data: |
Morphology: | epithelioid cells growing in monolayers; image; image |
Medium: | 100% Keratinocyte SFM + 0.2ng/ml hEGF + 15µg/ml pituitary extract |
Subculture: | seed out at ca. 0.3 x 106 cells in a T75 flask; split 1:4 - 1:8 every 5-8 days using Accutase |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | ca. 50-60 hours |
Harvest: | cell harvest of ca. 2-3 x 106 cells/75 cm2 |
Storage: | frozen with 90% Keratinocyte SFM, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in PCR assay |
Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV + |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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