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Cell line: | ETCC-016 |
DSMZ no.: | ACC 798 |
Species: | human (Homo sapiens) |
Cell type: | lung adenocarcinoma |
Origin: | established in 2011 from the pleural effusion of a 53-year-old man with advanced adenocarcinoma of the lung; described to carry increased copy numbers of the EGFR and MYC genes, harbour extensive LOH and to induce tumors in SCID mice |
Reference(s): | 18126 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
Depositor-specific restrictions: | Transfer to third parties needs to be negotiated with the depositor. |
| DSMZ Cell Culture Data: |
Morphology: | epithelioid cells growing in monolayers |
Medium: | 90% DMEM + 10% h.i. FBS |
Subculture: | seed out at ca. 1 x 106 cells/80 cm2 flask; split confluent culture 1:3 - 1:4 every second day using trypsin/EDTA |
Incubation: | at 37 °C with 10% CO2 |
Doubling time: | ca. 50 hours |
Harvest: | cell harvest of ca. 10-15 x 106 cells/175 cm2 |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in PCR assay |
Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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