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| Cell line: | MBU-TS1 |
| DSMZ no.: | ACC 800 |
| Species: | human (Homo sapiens) |
| Cell type: | keratinocytes |
| Origin: | established in 2011 from primary keratinocytes isolated from neonatal foreskin, transduced with retroviral vectors containing human telomerase and early SV40 region; carries additionally a FLAG epitope-tagged HRAS(V12) linked to IRES-driven GFP expression; MBU-TS1 is a xenograft-derived side line of MBU-T in Swiss nude mice |
| Reference(s): | 25648 |
| Biosafety level: | 2, GMO-S2 |
| Risk assessment: | Primary keratinocyte cells isolated from a neonatal foreskin were transduced with replication deficient amphotropic retroviral vectors containing a) the catalytic subunit of the human telomerase reverse transcriptase hTERT, b) SV40 early region, and c) constitutively active mutated HRASV12 genes for immortalization. Mutated HRAS exhibits strong oncogenic potential. |
| Permissions and restrictions: | A, B, D |
| Depositor-specific restrictions: | Transfer to third parties needs to be negotiated with the depositor. |
| | DSMZ Cell Culture Data: |
| Morphology: | epithelioid cells growing in monolayers; image; image |
| Medium: | 100% Keratinocyte SFM + 0.2 ng/ml hEGF + 15 µg/ml pituitary extract (no FBS added) |
| Subculture: | seed out at ca. 0.5 x 106 cells/80 cm2 flask; split 1:2 every 8-10 days using Accutase (rinse two times with PBS and incubate for about 15 min at 37°C) |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 100-140 hours |
| Harvest: | cell harvest of ca. 2 x 106 cells/175 cm2 |
| Storage: | frozen with 90% Keratinocyte SFM, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in PCR assay |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV + |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
