| | |
|---|
| Cell line: | MBU-TS4 |
| DSMZ no.: | ACC 801 |
| Species: | human (Homo sapiens) |
| Cell type: | keratinocytes |
| Origin: | established in 2011 from primary keratinocytes of a neonatal foreskin; cells were transduced with retroviral vectors containing human telomerase, SV40 early region, and epitope-tagged mutated HRAS(V12) linked to IRES-driven GFP expression; MBU-TS4 is a xenograft-derived side line of MBU-T in Swiss nude mice |
| Reference(s): | 25648 |
| Biosafety level: | 2, GMO-S2 |
| Risk assessment: | The primary keratinocyte cells were transduced with retroviral vectors containing a) human telomerase reverse transcriptase hTERT, b) SV40 early region, and c) constitutively active mutated HRASV12 genes for immortalization. Mutated HRAS exhibits strong oncogenic potential. |
| Permissions and restrictions: | A, B, D |
| Depositor-specific restrictions: | Transfer to third parties needs to be negotiated with the depositor. |
| | DSMZ Cell Culture Data: |
| Morphology: | epithelioid cells growing in monolayers; image; image |
| Medium: | 100% Keratinocyte SFM + 0.2 ng/ml hEGF + 15 µg/ml pituitary extract |
| Subculture: | seed out at ca. 1 x 106 cells/175 cm2 flask; split 1:3 - 1:4 every 6-8 days using Accutase (rinse two times with PBS and incubate for about 15 min at 37°C) |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 80-100 hours |
| Harvest: | cell harvest of ca. 3-4 x 106 cells/175 cm2 |
| Storage: | frozen with 90% Keratinocyte SFM, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in PCR assay |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV + |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
