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| Cell line: | MCC-60 |
| DSMZ no.: | ACC 815 |
| Species: | human (Homo sapiens) |
| Cell type: | colorectal cancer |
| Origin: | established in 2016 from the primary adenocarcinoma (TNM stage pT4b, pN1a, pM1; tumor grade G3; resection status R0) of the ascending colon of a 65-year-old man |
| Reference(s): | 18233 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | epithelioid cells growing adherently in monolayers |
| Medium: | 80-85% DMEM + 15-20% h.i. FBS |
| Subculture: | seed out at ca. 0.5 x 106 cells/80 cm2 flask; split confluent culture 1:4 once a week using trypsin/EDTA; if the cells grow slowly in the first week after thawing, change medium only |
| Incubation: | at 37 °C with 10% CO2 |
| Doubling time: | ca. 70-80 hours |
| Harvest: | cell harvest of ca. 5-7 x 106 cells/175 cm2 |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in PCR assay |
| Immunology: | to inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HTLV-I/-II -, MLV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
