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| Cell line: | MC-116 |
| DSMZ no.: | ACC 82 |
| Species: | human (Homo sapiens) |
| Cell type: | B cell lymphoma |
| Origin: | established from the pleural effusion of a patient with B cell lymphoma (undifferentiated lymphoma); cells were described in the literature to be EBV-negative, to carry the t(8;14) and to express mRNA of the proto-oncogene BCL2 |
| Reference(s): | 14731, 14730 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | round to elongated, lymphoblastoid, single or clustered cells in suspension; image; image; image; image; image |
| Medium: | 80-85% RPMI 1640 + 15-20% h.i. FBS |
| Subculture: | maintain at 1-2 x 106 cells/ml; split ratio of 1:2 to 1:3; seed out at ca. 1 x 106 cells/ml |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | about 24-48 hours |
| Harvest: | maximal density at 2.5 x 106 cells/ml |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology: | CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD138 -, HLA-DR +, sm/cyIgM +, sm/cyIgG -, sm/cykappa -, sm/cylambda + |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human near diploid karyotype with 1.5% polyploidy - 46(37-46)<2n>XY, dup(1)(q21q32), t(8;14)(q24;q32), del(10)(q23) |
| Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | Growing culture
(please inquire for exact delivery time) | | 1040,- € | | Frozen culture | | 520,- € | | DNA isolated from cell line (25 µg) | | 650,- € | | DNA isolated from cell line (5 µg) | | 160,- € |
see price list |
| | Print data sheet |
