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| Cell line: | EL-4 |
| DSMZ no.: | ACC 831 |
| Species: | murine (mouse) (Mus musculus) |
| Cell type: | T cell lymphoma |
| Origin: | established from a Dimethyl-benzanthracene induced lymphoma in a C57BL/6N mouse |
| Reference(s): | 18170, 18171, 40928, 40929 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| Depositor-specific restrictions: | Transfer to third parties needs to be negotiated with the depositor. |
| | DSMZ Cell Culture Data: |
| Morphology: | small, rounded to oval or multiform cells growing mostly singly in suspension; some cells exhibit tiny hairs or fringes |
| Medium: | 95% RPMI 1640 + 5% h.i. FBS |
| Subculture: | seed out at ca. 0.3-0.5 x 106 cells/ml; maintain between 1.0 and 1.5 x 106 cells/ml; split saturated culture 1:3-1:5 every 2-3 days |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 15-20 hours |
| Harvest: | maximal density at about 1.8 x 106 cells/ml |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in PCR assay |
| Species validation: | Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species. |
| Viruses: | PCR: MLV + |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
