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Cell line: | U2OS#18-XRCC3-6A/X3+ |
DSMZ no.: | ACC 842 |
Species: | human (Homo sapiens) |
Cell type: | osteosarcoma |
Origin: | Genetical modification of U2OS#18 (DSMZ ACC 832, a reporter cell line for studying sister chromatid recombination, SCR). This cell line belongs to the series of genetically disrupted RAD51 paralogs with reversion of the disruption by retroviral transfection with the functional gene (the non-complemented sister cell line is ACC 837). Cells are described in the literature to be puromycin and hygromycin resistant |
Reference(s): | 18169, 18205, 18186 |
Biosafety level: | 1, GMO-S1 |
Risk assessment: | genetically modified organism, according to German and European legislation allocated into group S1 |
Permissions and restrictions: | A, D |
Depositor-specific restrictions: | Transfer to third parties needs to be negotiated with the depositor. |
| DSMZ Cell Culture Data: |
Morphology: | epithelioid cells growing adherently as monolayer; image; image; image |
Medium: | 90% DMEM + 10% h.i. FBS + (additionally) 2 mM L-glutamine (to verify antibiotic resistance, 1 µg/ml puromycin and/or 150 µg/ml hygromycin should be added) |
Subculture: | seed out at 1 x 106 cells/80 cm2; split 1:10 to 1:20 once or twice a week using trypsin/EDTA |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | ca. 35 hours |
Harvest: | cell harvest of ca. 14 x 106 cells/80 cm2 |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in PCR assay |
Immunology: | to inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Molec. Genetics: | frameshift mutations in exon 5 of XRCC3 were verified by sequence analysis and forced expression of XRCC3 in the retrovirally transfected cell line was confirmed by RQ-PCR analysis |
Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV1/2 -, MLV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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