| | |
|---|
| Cell line: | HEK293-DR-GFP-RAD51B-9 |
| DSMZ no.: | ACC 845 |
| Species: | human (Homo sapiens) |
| Cell type: | embryonal kidney |
| Origin: | Genetic modification of HEK293-DR-GFP (DSMZ ACC 844, a hprtDR-GFP reporter cell line for studying chromosomal double-strand repair (DR)). This cell line carries a genetic disruption of RAD51B by frameshifting mutations in exon 3 (targeted by CRISPR-CAS9) and is puromycin resistant. The wild-type allele is no longer present. A sister cell line with the retrovirally reconstituted RAD51B gene is available as ACC 850 |
| Reference(s): | 18173, 18205 |
| Biosafety level: | 1, GMO-S1 |
| Risk assessment: | genetically modified organism, according to German and European legislation allocated into group S1 |
| Permissions and restrictions: | A, D |
| Depositor-specific restrictions: | Transfer to third parties needs to be negotiated with the depositor. |
| | DSMZ Cell Culture Data: |
| Morphology: | adherent fibroblastoid cells growing as monolayer with formation of islets, some cells showing small extensions; image; image; image |
| Medium: | 90% DMEM + 10% h.i. FBS + (additionally) 2 mM L-glutamine (to sustain genetic stability cells should be maintained, at least periodically, with 0.5 µg/ml puromycin) |
| Subculture: | culture vessels should be coated with 0.01% poly-L-lysine solution; seed out at 1 x 106 cells/25 cm2; split subconfluent culture 1:2 to 1:3 every 2-3 days using trypsin/EDTA; optimal density around 4 x 106 cells/25 cm2 |
| Incubation: | at 37 °C with 10% CO2 |
| Doubling time: | ca. 48 hours |
| Harvest: | cell harvest of ca. 8 x 106 cells/25 cm2 flask |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in PCR assay |
| Immunology: | to inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Molec. Genetics: | frameshift mutation in exon 3 of RAD51B was verified by sequence analysis |
| Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV1/2 -, MLV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
