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| Cell line: | MB-021 |
| DSMZ no.: | ACC 85 |
| Species: | insect - cabbage moth (Mamestra brassicae) (Mamestra brassicae) |
| Cell type: | larval hemocytes |
| Origin: | established from the larvae of the butterfly Mamestra brassicae (family Noctuidae, subfamily Noctuinae) (cabbage moth; Kohleule) |
| Reference(s): | 14712 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | polymorphic round cells, partly adherent; image; image |
| Medium: | 85% Grace's Insect Medium (Modified) + 15% h.i. FBS |
| Subculture: | split confluent cultures 1:2 about twice a week, but with using trypsin (cells grow partly adherent, but can be detached without trypsin); maintain at room temperature (about 20 °C) without CO2 exposure ; confluent cultures can be stored at 4 °C for up to one week without loss of viability |
| Incubation: | at 20 °C without CO2 (at room temperature, keep lid of flask closed) |
| Harvest: | about 5-7 x 106 cells/80 cm2 |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization assays |
| Species validation: | Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mamestra brassicae species. |
| Viruses: | PCR: SMRV - |
| Supplied as: | | Delivery form | | Prices | | Growing culture
(please inquire for exact delivery time) | | 1040,- € | | Frozen culture | | 520,- € | | DNA isolated from cell line (25 µg) | | 650,- € | | DNA isolated from cell line (5 µg) | | 160,- € |
see price list |
| | Print data sheet |
