| | |
|---|
| Cell line: | HEK293-DR-GFP-RAD51D-16/D+ |
| DSMZ no.: | ACC 852 |
| Species: | human (Homo sapiens) |
| Cell type: | embryonal kidney |
| Origin: | Genetic modification of HEK293-DR-GFP-RAD51D-16 (DSMZ ACC 847) which belongs to a series of genetically disrupted RAD51 paralogs. Here, in ACC 852, the RAD51D gene is functionally reconstituted by retroviral transfection. Cells are described in the literature to be puromycin and hygromycin resistant |
| Reference(s): | 18205, 18173 |
| Biosafety level: | 1, GMO-S1 |
| Risk assessment: | genetically modified organism, according to German and European legislation allocated into group S1 |
| Permissions and restrictions: | A, D |
| Depositor-specific restrictions: | Transfer to third parties needs to be negotiated with the depositor. |
| | DSMZ Cell Culture Data: |
| Morphology: | adherent fibroblastoid cells growing as monolayer; image; image; image |
| Medium: | 90% DMEM + 10% h.i. FBS + (additionally) 2 mM L-glutamine (to sustain genetic stability cells should be maintained, at least periodically, with 0.5 µg/ml puromycin and/or 100 µg/ml hygromycin) |
| Subculture: | culture vessels should be coated with 0.01% poly-L-lysine solution; seed out at 1 x 106 cells/80 cm2; split 1:10 to 1:20 once or twice a week using TrypleExpress (trypsin/EDTA may not be efficient) |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 30 hours |
| Harvest: | cell harvest of ca. 20 x 106 cells/80 cm2 flask |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in PCR assay |
| Immunology: | to inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Molec. Genetics: | frameshift mutation in exon 1 of RAD51D was verified by sequence analysis and forced expression of RAD51D in the retrovirally transfected cell line was confirmed by RQ-PCR analysis |
| Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV1/2 -, MLV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
