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| Cell line: | MUG-MEL2 |
| DSMZ no.: | ACC 855 |
| Species: | human (Homo sapiens) |
| Cell type: | melanoma |
| Origin: | established in 2014 from a cutaneous metastasis of a 48-year-old man with highly aggressive ulcerated melanoma after chemotherapy. Cells are described to be moderately hyperdiploid, carry a mutation in the N-Ras-gene (pQ61R) but not in the BRAF. Further described to be tumorigenic in nude mice and able to grow in 3D using hydrogel |
| Reference(s): | 18175 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| Depositor-specific restrictions: | Transfer to third parties needs to be negotiated with the depositor. |
| | DSMZ Cell Culture Data: |
| Morphology: | elongate or triangular dendritic cells growing adherently as monolayer; cells are highly pigmented and sedimented cells have a brown color |
| Medium: | 80% RPMI 1640 + 20% h.i. FBS + 2 mM glutamine |
| Subculture: | seed out at 1 x 106 cells/80 cm2;
split confluent culture 1:4 to 1:6 every 4-6 days using trypsin/EDTA |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 48 hours |
| Harvest: | cell harvest of ca. 5 x 106 cells/80 cm2 flask |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in PCR assay |
| Immunology: | to inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Molec. Genetics: | Genomic sequencing and/or ARMS assay show that the cell line carries the NRAS Q61R mutation (mu/wt), but no BRAF V600 mutation |
| Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV1/2 -, MLV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
